Protective effects of panax quinquefolium 20s-protopanaxtriol saponins on ventricular remodeling in rats with pressure overloaded hypertrophic myocardium / 中华老年医学杂志

ObjectiveTo investigate the protective effects of panax quinquefolium 20s-protopanaxtriol saponins (PQTS) on ventricular remodeling in rats with pressure overloaded hypertrophic myocardium.Methods Wister rats were randomly divided into operation,model,positive captopril,and low,moderate,high PQTS groups.The model of pressure overload-induced ventricular remodeling was established through the method of rat's abdominal aorta deligation.After 6 weeks of PQTS treatment ( 12.5,25.0 and 50.0 mg · kg-1 · d-1,i.p),myocardial morphological and hemodynamic parameters were determined.The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD)in serum,and the concentrations of prostacycline (PGI2),thromboxane A2 (TXA2),endothelium (ET) and angiotensin Ⅱ( Ang Ⅱ ) in plasma were also determined.ResultsCompared with remodeling group,PQTS could inhibit myocardial pathological changes,decrease significantly ventricular weight and cardiac coefficient,increase significantly systolic blood pressure,diastolic blood pressure,mean arterial pressure,left ventricular systolic pressure and the maximum left ventricular pressure rising and dropping rates(dp/dtmax),reduce the heart rate and left ventricular end-diastolic pressure in ventricular remodeling rats.PQTS could also decrease the content of MDA and enhance significantly activity of SOD in serum.In addition,PQTS could decline the contents of ET,Ang Ⅱ and TXA2 in plasma while increase significantly the content of PGI2 in plasma and PGI2/TXA2 ratio(P＜0.05 or P＜0.01).ConclusionsPQTS has protective effects on ventricular remodeling through improving systolic and diastolic function in ventricular remodeling rats,increasing anti-oxidase activity,reducing the damage of free radicals and vasoactive substance onmyocardium,and correcting disequilibrium of PGI2/TXA2 in ventricular remodeling rats.

Acanthopanax senticosus saponins ameliorates oxidative damage induced by hydrogen peroxide in neonatal rat cardiomyocytes / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effect of Acanthopanax senticosus saponins (ASS) on oxidative damage induced by hydrogen peroxide (H2O2 ) in cardiomyocytes.</p><p><b>METHOD</b>The cardiomyocytes were induced to oxidative damaged by exposed to H2O2. We evaluated the level of oxidative injury through morphology change, the survival rate, the activity of lactate dehydrogenase (LDH) and the content of cellular malondialdehyde (MDA). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and the content of reductive glutathione (GSH) of cardiomyocytes were also determined.</p><p><b>RESULT</b>When the cardiomyocytes were exposed to H2O, (50, 100, 200 micromol x L(-1)) for deigned time, the percentage of survival cells was down significantly (P < 0.01 or P < 0.001), and the activity of LDH and the content of MDA were increased markedly (P < 0.05, P < 0.01 or P < 0.001). These results show that the cells were subjected to oxidative damage. Treatment with ASS (600 mg x L(-1)) prior to H2O2 exposure could increase cell viability significantly, lessen cardiomyocyte morphological damaged change, and decrease LDH activity (1687.40 +/- 97.51) U x mL(-1) in media and cellular MDA content (16.50 +/- 2.66) nmol x mg(-1) markedly (P < 0.01 and P < 0.05). Furthermore, the activities of SOD (89.55 +/- 6.93) U x mg(-1), GSH-Px (845.87 +/- 63.76) mU x mg(-1), CAT (93.07 +/- 10.40) U x mg(-1) and the content of GSH (8.91 +/- 1.06) micromol x mg(-1) of cardiomyocytes were also raised by 600 mg x L(-1) l ASS (P < 0.05).</p><p><b>CONCLUSION</b>Taken together, the study implicate that ASS protects cardiomyocytes against oxidative-stress injury induced by H2O2 through reduction of lipid peroxidation and enhancement of the activity of antioxidant defense.</p>

Protective effect and mechanism of IPQDS on acute myocardial infarction in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the protective effect of Injection of Panax quinquefolium diolsaponins (IPQDS) and its mechanism on acute myocardial infarction in rats.</p><p><b>METHOD</b>The acute myocardial infarct model was prepared by left anterior descending coronary occulusion for 24 hours in open chest anesthetized rats. The myocardial infarct size (MIS) was calculated. The activities of serum creatine hosphokinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the contents of malondialdehyde (MDA) and nitric oxide (NO) were determined. Blood was collected to observe low shearing specific viscosity, middle shearing specific viscosity and high shearing specific viscosity of whole blood and plasma viscosity. At the same time, the platelet aggregation rate and platelet adherence rate were also determined.</p><p><b>RESULT</b>In rats treated by IPQDS (in a dosage of 12.5, 25 and 50 mg x kg(-1) d, i.p. 3d ), the MIS was significantly reduced. The activities of serum CK, LDH and AST, and the content of serum MDA were declined. The activities of SOD and GSH-Px, and the content of NO were increased markedly. In addition, low shearing specific viscosity, middle shearing specific viscosity and high shearing specific viscosity of whole blood and plasma viscosity as well as platelet aggregation rate were also declined significantly. But platelet adherence rate had no significant change.</p><p><b>CONCLUSION</b>IPQDS has a protective effect on acute myocardial ischemia, which may be related to increasing activity of antioxidase in body, scavenging the damage of peroxidation from oxygen free radicals, decreasing the viscosity of blood and plasma and preventting thrombosis etc.</p>

Protective effects of Diemailing Injection on experimental myocardial infarction in rats and its mechanism / 吉林大学学报(医学版)

Objective To observe the protective effects of Diemailing Injection(DMLI) on experimental myocardial infarction in rats and its mechanism.Methods The experimental myocardial infarction model was induced by left anterior descending coronary occulusion for 24 h in rats.The rats were randomly divided into sham group,myyocardial infaction model group,DMLI groups with different doses(2.5,5.0,10.0 mL?kg~(-1))(n=20).The changes of myocardial infarction size(MIS),aspartate aminotransferase(AST),actate dehydrogenase(LDH),creatine phosphokinase(CK),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities and malondialdehyde(MDA) content in serum,endothelin(ET) and angiotensinⅡ(AngⅡ) levels in plasma,and low shearing specific viscosity,middle shearing specific viscosity and high shearing specific viscosity of blood and specific viscosity of plasma were determined.At the same time,myocardial free fatty acid(FFA) contents of infarction and noninfarction area were determined.Results In rats treated by DMLI(in doses of 2.5,5.0 and 10.0 mL?kg~(-1) i.v after coronary occulusion),the MIS was significantly reduced(P

Protective effects of Diemailing Injection on myocardial ischemia-reperfusion injury in rats / 吉林大学学报(医学版)

Objective To observe the protective effects of Diemailing Injection (DMLI) on myocardial ischemia-reperfusion injury in rats. Methods The myocardial ischemia-reperfusion model was induced by 30 min left anterior descending coronary occulusion and 24 h reperfusion in open-chest anesthetized rats. The changes of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), MB isoenzyme of creatine kinase (CK-MB), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) contents in serum, and prostacycline (PGI2) and thromboxane A2 (TXA2) levels in plasma were determined. Results In rats treated by DMLI (with dose of 2. 5, 5 and 10 mL ? kg-1 i. v. at 30 min after coronary occulusion), the myocardial ischemia size (MIS) was significantly reduced, the AST, LDH and CK-MB activities in serum and the TXA2 level in plasma were declined, while PGI2 level in plasma and PGI2/TXA2 ratio were increased significantly. In addition, the LPO content in serum declined, SOD and GSH-Px activities in serum were increased markedly. Conclusion DMLI has protective effects on myocardial ischemia-reperfusion injury through improving free radicals metabolism, decreasing TXA2 level in plasma, increasing PGI2 level in plasma and PGI2/TXA2 ratio.

Mechanism of action of PQDS on anti-ventricular remodeling in rats / 吉林大学学报(医学版)

Objective To observe the mechanism of action of panax quinquefolium diolsaponins (PQDS) on anti-ventricular remodeling in rats. Methods The model of pressure-loading ventricular remodeling was established through the method of rat’s abdominal aorta deligation. The male Wistar rats were divided into 5 groups,including sham operation group,remodeling model group,Benazepril group,and low and high dose groups of PQDS. The rats were treated with PQDS (with dose of 50 and 100 mg?kg-1 i.g) for 6 weeks. The rats in sham operation group and remodeling model group were treated with normal sodium (with dose of 10 mL?kg-1?d-1 i.g) for 6 weeks. The rats in masculine medicine group were treated by Benazepril (with dose of 10 mg?kg-1 i.g) for 6 weeks. After 6 weeks of treatment,myocardial morphological parameters,the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in serum,the contents of prostacycline (PGI2),thromboxane A2 (TXA2),nitric oxide (NO) and angiotensinⅡ (AngⅡ) in plasma were determined. At the same time,the contents of endothelium (ET) in plasma and myocardium were also determined. Results Compared with remodeling model group,the ventricular weight and cardiac coefficient were decreased significantly in the rats treated with PQDS (P0.05). Conclusion PQDS has protective effects on ventricular remodeling through increasing anti-oxidase activity,reducing the damage of free radicals and vasoactive substance on myocardium and correcting disequilibrium of PGI2/TXA2 in ventricular remodeling etc.

Effect of Acanthopanax senticosus saponins on organ blood flow in anesthetized dogs / 吉林大学学报(医学版)

Objective To study the effect of Acanthopanax senticosus saponins (ASS) on organ blood flow and vascular resistance in anesthetized dogs.Methods The cerebral blood flow(CBF),cerebral vascular resistance(CVR),peripheral blood flow(PBF),peripheral vascular resistance(PVR),blood pressure(SDP and DBP) and heart rate(HR) were measured using the MF-27 electromagnetic flowmeter.Results ASS(15 and 30 mg?kg~(-1) iv perfusion for 60 min) augmented CBF and PBF,decreased CVR and PVR within 180 min and slowed down HR at 60-180 min.In addition,ASS(30 mg?kg~(-1) iv perfusion for 60 min) could obviously reduce SBP and DBP.Conclusion Protective effect of ASS on experimental cerebral ischemia may be related to improving cerebral circulation and increasing cerebral blood supplyment.

Effects of ginseng fruit saponins(GFS)on coronary circulation and myocardial oxygen metabolism in dogs with acute myocardial infarction / 吉林大学学报(医学版)

Objective To study the effects of ginseng fruit saponins(GFS)on the coronary circulation and cardial oxygen metabolism in dogs with acute myocardial infarction.Methods The acute myocardial infarction model was induced by ligating the left anterior descending coronary of dogs for 6 h.90 min after treatment with GFS,the blood flow of coronary artery was determined by using electromagnetism flowmeter with MP-27 type.At the same time,the blood oxygen contents of artery and vein were determined by using analysis instrument of blood gas with CORNIHG 178 type.Results After treated by GFS(in a dosage of 5 and 10 mg?kg~(-1) iv drip for 90 min),the myocardial blood flow(MBF) was increased and coronary vascular resistance(CVR) was decreased significantly.Meanwhile the cardiac oxygen consumption and myocardial oxygen utilization rate were also decreased,but cardiac oxygen consumption index had not changed significantly.Conclusion GFS has protective effects on acute myocardial ischemia through improving the coronary circulation,increasing myocardial blood supply and decreasing cardiac oxygen consumption and myocardial oxygen utilization rate.

Effect of 20(S)-Protopanaxadiol on SMMC-7721 human liver cancer in vivo and in vitro / 中国药理学通报

Aim To study the anti-tumor effect of 20(S)-Protopanaxadiol(PPD)at different concentration on human liver cell line SMMC-7721 in vivo and in vitro.Methods The subcutaneous transplantable tumor model of human liver cancer in nude mice was established and the anti-tumor effect was calculated.Cell growth rate was determined with MTT assay.The apoptosis was analyzed by FITC-AnnexinⅤ/PI and Hoechst33342 staining method,and the activity of Caspase-3 was detected.Results In vivo,PPD could obviously inhibit the growth of transplanted tumor.In vitro,PPD induced inhibition of human liver cancer SMMC-7721 cells was time-dependent and dose-dependent.The apoptotic body was observed by Hoechst33342 staining.PPD could induce cell apoptosis of SMMC-7721,and the increase of Caspase-3 activity was observed in each PPD group.Conclusion PPD could inhibit the growth of human liver cancer SMMC-7721 cells in vivo and in vitro by up-regulating the activity of Caspase-3 and inducing the cell apoptosis.

Effects of TFAD on the blood-liquid metabolism in experimental hyperlipoproteinemia rats / 中国药理学通报

Aim To study the effects of totle flavone of allium cepa L.var agrogatum Don(TFAD)on the blood-liquid metabolism in experimental hyperlipoproteinemia rats.Methods The model of experimental hyperlipoproteinemia was established by giving hyperlipid diet to Wistar rats.After the model was established,the rats were treated by TFAD.Then TC、TG、LDL-C、HDL-C of serum,MDA and SOD activties of serum and liver,TXA2 and PGI2 of plasma,blood viscosity were mesured,and pathology of liver was observed.Results After treated by TFAD(25～100 ?g?g-1 po for 21 days),TC、TG、LDL-C、AI、TC/HDL-C and MDA were reduced remarkably,HDL-C and the activity of SOD increased,PGI2 in plasma rose while TXA2 decreased,the blood viscosity of whole blood was depressed significantly,meanwhile the fatty degeneration of hepatic cells was relieved.Conclusions The results suggest that TFAD can adjust the blood-liquid metabolism of experimental hyperlipoproteinemia rats and restrain fatty deposition in liver which may be related to TFAD's function of preventing lipoprotein peroxidization,maitaining physiologic equilibrium of PGI2/TXA2,and ameliorating the abnormity of blood rheology.

Protection of Shexiang Antithrombosis Pill on experimental cerebral ischemia in rats / 中草药

Object To observe the protective effect of Shexiang Antithrombosis Pill (SATP) on experimental cerebral ischemia in rats. Methods In cerebral ischemia model induced by bilateral common carotid artery clamping and controlled hemorrhage to a mean arterial pressure of 6.7 kPa (50 mmHg), cerebral water content, SOD activity, MDA, Ca 2+ , and LA contents were measured and the ischemic tissue pathology was observed. Results SATP can decrease cerebral water content, lessen pathological change, increase SOD activity and decrease MDA, Ca 2+ , and LA contents. Effect of water pill is better than that of honey pill. Conclusion SATP can protect the cerebral tissue from ischemia injury by suppressing lipid peroxidation. This effect may be related to increasing antioxidase activity and decreasing acidosis of LA and overload of calcium in cell.

Effect of 20S-protopanaxadiol saponins on blood lipid metabolism and antioxidative activity in hyperlipidemia rats / 中草药

Object To observe the effect of 20S-protopanaxadiol saponins from Panax quinquefolium (PPDS) on total cholesterol, lipoprotein cholesterol metabolism and antioxidative activity in experimental hyperlipidemia rats. Methods The total cholesterol (TC), lipoprotein cholesterol, and lipid peroxidation (LPO) contents, prostaglandin I 2 (PGI 2), thromboxane A 2 (TXA 2) levels, superoxide dismutase (SOD) activity and blood viscosity were measured in hyperlipidemia rats which have been given PPDS 25, 50, 100 mg/(kg?d) by ip, continuously for 12 days. In addition, fat accumulation in liver was observed. Results Triglyceride (TG), TC, low density lipoprotein cholesterol (LDL-c) in serum, TXA 2 in plasma, LPO in serum and liver, and blood viscosity were decreased significantly; and high density lipoprotein cholesterol (HDL-c) in serum, PGI 2 in plasma, and SOD in serum and liver were significantly increased by given PPDS [50, 100 mg/(kg?d)] in experimental hyperlipidemia rats. Moreover, PPDS can decrease TC/HDL-c and LDL-c/HDL-c ratio, increase PGI 2/TXA 2 ratio, and inhibit fat accumulation in liver. Conclusion PPDS could inhibit arteriosclerosis by improving cholesterol and lipoprotein-cholesterol metabolism, suppressing LPO, and increasing the activity of SOD.